pyfakefs (homepage) does what you want – a fake filesystem; it’s third-party, though that party is Google. See How to replace file-access references for a module under test for discussion of use.
For mocking, unittest.mock is the standard library for Python 3.3+ (PEP 0417); for earlier version see PyPI: mock (for Python 2.5+) (homepage).
Terminology in testing and mocking is inconsistent; using the Test Double terminology of Gerard Meszaros, you’re asking for a “fake”: something that behaves like a filesystem (you can create, open, and delete files), but isn’t the actual file system (in this case it’s in-memory), so you don’t need to have test files or a temporary directory.
In classic mocking, you would instead mock out the system calls (in Python, mock out functions in the os module, like os.rm and os.listdir), but that’s much more fiddly.