Will pandas dataframe object work with sklearn kmeans clustering?

Assuming all the values in the dataframe are numeric,

# Convert DataFrame to matrix
mat = dataset.values
# Using sklearn
km = sklearn.cluster.KMeans(n_clusters=5)
km.fit(mat)
# Get cluster assignment labels
labels = km.labels_
# Format results as a DataFrame
results = pandas.DataFrame([dataset.index,labels]).T

Alternatively, you could try KMeans++ for Pandas.