All of your bitfield members are signed 1-bit integers. On a two’s complement system, that means they can represent only either 0 or -1. Use uint8_t if you want 0 and 1:
struct Bits
{
uint8_t b0:1, b1:1, b2:1, b3:1, b4:1, b5:1, b6:1, b7:1;
};