Use the datetime.timedelta class:
>>> from datetime import datetime, timedelta
>>> then = datetime.now() - timedelta(hours = 2)
>>> now = datetime.now()
>>> (now - then) > timedelta(days = 1)
False
>>> (now - then) > timedelta(hours = 1)
True
Your example could be written as:
if (datetime.now() - self.timestamp) > timedelta(seconds = 100)
or
if (datetime.now() - self.timestamp) > timedelta(minutes = 100)