Pytest use same fixture twice in one function

An alternative is just to copy the fixture function. This is both simple and correctly handles parameterized fixtures, calling the test function with all combinations of parameters for both fixtures. This example code below raises 9 assertions:

import pytest

@pytest.fixture(params=[0, 1, 2])
def first(request):
    return request.param

second = first

def test_double_fixture(first, second):
    assert False, '{} {}'.format(first, second)