The pygame module can be used to play midi files.
http://www.pygame.org/docs/ref/music.html
See the example here:
http://www.daniweb.com/software-development/python/code/216979
a whole bunch of options available at:
http://wiki.python.org/moin/PythonInMusic
and also here which you can modify to suit your purpose:
http://xenon.stanford.edu/~geksiong/code/playmus/playmus.py