You can provide loader in Environment from that list
from jinja2 import Environment, BaseLoader
rtemplate = Environment(loader=BaseLoader).from_string(myString)
data = rtemplate.render(**data)
Edit: The problem was with myString, it has {% include 'test.html' %} and Jinja2 has no idea where to get template from.
UPDATE
As @iver56 kindly noted, it’s better to:
rtemplate = Environment(loader=BaseLoader()).from_string(myString)