You can add a shebang line the to the top of the script:
#!/usr/bin/env python2.7
But that will only work when executing as ./my_program.py.
If you execute as python my_program.py, then the whatever Python version that which python returns will be used.
In re: to virtualenv use: virtualenv -p /usr/bin/python3.2 or whatever to set it up to use that Python executable.