Checking on a thread / remove from list

As TokenMacGuy says, you should use thread.is_alive() to check if a thread is still running. To remove no longer running threads from your list you can use a list comprehension:

for t in my_threads:
    if not t.is_alive():
        # get results from thread
        t.handled = True
my_threads = [t for t in my_threads if not t.handled]

This avoids the problem of removing items from a list while iterating over it.