AssertionError when threading in Python

You want to specify the target keyword parameter instead:

t1 = threading.Thread(target=analysis("samplequery"))

You probably meant to make analysis the run target, but 'samplequery the argument when started:

t1 = threading.Thread(target=analysis, args=("samplequery",))

The first parameter to Thread() is the group argument, and it currently only accepts None as the argument.

From the threading.Thread() documentation:

This constructor should always be called with keyword arguments. Arguments are:

• group should be None; reserved for future extension when a ThreadGroup class is implemented.
• target is the callable object to be invoked by the run() method. Defaults to None, meaning nothing is called.