You can do it by putting it inside a frame, forcing the frame to a fixed size by deactivating size propagation and configuring the entry to stick to the frame borders. Should work in a similar way with pack, too.
import Tkinter # tkinter with small t for python 3
#import ttk # nicer widgets
root = Tkinter.Tk()
mainFrame = Tkinter.Frame(root)
mainFrame.grid()
button = Tkinter.Button(mainFrame, text="dummy")
button.grid()
entryFrame = Tkinter.Frame(mainFrame, width=454, height=20)
entryFrame.grid(row=0, column=1)
# allow the column inside the entryFrame to grow
entryFrame.columnconfigure(0, weight=10)
# By default the frame will shrink to whatever is inside of it and
# ignore width & height. We change that:
entryFrame.grid_propagate(False)
# as far as I know you can not set this for x / y separately so you
# have to choose a proper height for the frame or do something more sophisticated
# input entry
inValue = Tkinter.StringVar()
inValueEntry = Tkinter.Entry(entryFrame, textvariable=inValue)
inValueEntry.grid(sticky="we")
root.mainloop()