The best way to do this is probably to call the input and output files as arguments for the python script:
import sys
inFile = sys.argv[1]
outFile = sys.argv[2]
Then you can read in all your data, do your manipulations, and write out the results:
with open(inFile,'r') as i:
lines = i.readlines()
processedLines = manipulateData(lines)
with open(outFile,'w') as o:
for line in processedLines:
o.write(line)
You can call this program by running python script.py input_file.txt output_file.txt
If you absolutely must pipe the data to python (which is really not recommended), use sys.stdin.readlines()