Simple:
$('div').not('#myid');
Using .not() will remove elements matched by the selector given to it from the set returned by $('div').
You can also use the :not() selector:
$('div:not(#myid)');
Both selectors do the same thing, however :not() is faster, presumably because jQuery’s selector engine Sizzle can optimise it into a native .querySelectorAll() call.