You can call pack_forget to remove a widget (if you use pack to add it to the window).
Example:
from tkinter import *
root = Tk()
b = Button(root, text="Delete me", command=lambda: b.pack_forget())
b.pack()
root.mainloop()
If you use pack_forget, you can later show the widget again calling pack again. If you want to permanently delete it, call destroy on the widget (then you won’t be able to re-add it).
If you use the grid method, you can use grid_forget or grid_remove to hide the widget.