Can we have assignment in a condition?

Why not try it out?

>>> def some_func():
...   return 2
... 
>>> if (a = some_func()):
  File "<stdin>", line 1
    if (a = some_func()):
          ^
SyntaxError: invalid syntax

So, no.

Update: This is possible (with different syntax) in Python 3.8

if a := some_func():