How can I split a column of tuples in a Pandas dataframe?

You can do this by doing pd.DataFrame(col.tolist()) on that column:

In [2]: df = pd.DataFrame({'a':[1,2], 'b':[(1,2), (3,4)]})

In [3]: df
Out[3]:
   a       b
0  1  (1, 2)
1  2  (3, 4)

In [4]: df['b'].tolist()
Out[4]: [(1, 2), (3, 4)]

In [5]: pd.DataFrame(df['b'].tolist(), index=df.index)
Out[5]:
   0  1
0  1  2
1  3  4

In [6]: df[['b1', 'b2']] = pd.DataFrame(df['b'].tolist(), index=df.index)

In [7]: df
Out[7]:
   a       b  b1  b2
0  1  (1, 2)   1   2
1  2  (3, 4)   3   4

Note: in an earlier version, this answer recommended to use df['b'].apply(pd.Series) instead of pd.DataFrame(df['b'].tolist(), index=df.index). That works as well (because it makes a Series of each tuple, which is then seen as a row of a dataframe), but it is slower / uses more memory than the tolist version, as noted by the other answers here (thanks to denfromufa).