Can’t remove a folder with os.remove (WindowsError: [Error 5] Access is denied: ‘c:/temp/New Folder’)

os.remove requires a file path, and raises OSError if path is a directory.

Try os.rmdir(folder+'New Folder')

Which will:

Remove (delete) the directory path. Only works when the directory is empty, otherwise, OSError is raised.

Making paths is also safer using os.path.join:

os.path.join("c:\\", "temp", "new folder")