How to use comparison and ‘ if not’ in python?

You can do:

if not (u0 <= u <= u0+step):
    u0 = u0+ step # change the condition until it is satisfied
else:
    do sth. # condition is satisfied

Using a loop:

while not (u0 <= u <= u0+step):
   u0 = u0+ step # change the condition until it is satisfied
do sth. # condition is satisfied