Add display:block; to your Iframe style like so:
.border iframe {
border: none;
width: 300px;
height: 100px;
margin: 0;
padding: 0;
opacity: 0.8;
display:block; /* Add this */
}
Iframe is an Inline Frame, meaning that it is an inline element, which like the img tag, can have issues with whitespace. Setting display:block; on it turns the Iframe into a block element (like a div), which will remove the whitespace issue.