Bash – How to remove all white spaces from a given text file?

$ man tr
NAME
    tr - translate or delete characters

SYNOPSIS
    tr [OPTION]... SET1 [SET2]

DESCRIPTION
   Translate, squeeze, and/or delete characters from standard 
   input, writing to standard output.

In order to wipe all whitespace including newlines you can try:

cat file.txt | tr -d " \t\n\r" 

You can also use the character classes defined by tr (credits to htompkins comment):

cat file.txt | tr -d "[:space:]"

For example, in order to wipe just horizontal white space:

cat file.txt | tr -d "[:blank:]"